6O3Y
Crystal structure of yeast Nrd1 CID in complex with Sen1 NIM3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-23 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 98.671, 103.046, 115.365 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.044 - 2.799 |
R-factor | 0.24 |
Rwork | 0.237 |
R-free | 0.30000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3clj |
RMSD bond length | 0.008 |
RMSD bond angle | 1.101 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.000 |
High resolution limit [Å] | 2.799 | 2.800 |
Rmerge | 0.147 | 0.488 |
Number of reflections | 14439 | |
<I/σ(I)> | 7.3 | |
Completeness [%] | 97.1 | |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | sodium citrate, lithium chloride, PEG 6000 |