6O3V
Crystal structure for RVA-VP3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2017-08-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 236.706, 236.706, 349.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.175 - 3.500 |
| R-factor | 0.2307 |
| Rwork | 0.229 |
| R-free | 0.26440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.871 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3235: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.175 | 3.625 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rpim | 0.052 | |
| Number of reflections | 71574 | 5807 |
| <I/σ(I)> | 4.5 | |
| Completeness [%] | 97.7 | |
| Redundancy | 14.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293.15 | Protein conc ~ 7 mg/ml 0.2 M Lithium Sulphate 0.1 M Sodium citrate pH 5.5 15% Ethanol |
