6O3T
Structural basis of FOXC2 and DNA interactions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.365, 41.812, 82.654 |
| Unit cell angles | 90.00, 98.30, 90.00 |
Refinement procedure
| Resolution | 36.377 - 3.060 |
| R-factor | 0.2349 |
| Rwork | 0.233 |
| R-free | 0.26830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5x07 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.429 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.150 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rpim | 0.065 | 0.233 |
| Number of reflections | 8013 | |
| <I/σ(I)> | 12.9 | |
| Completeness [%] | 96.8 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298.15 | 30% PEG MME 2000, 100 mM sodium acetate pH 4.6, 200 mM ammonium sulfate, 10 mM BME |
