6O3B

Crystal structure of Frizzled 7 CRD in complex with F6 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03327
Spacegroup name	C 1 2 1
Unit cell lengths	139.642, 85.848, 104.103
Unit cell angles	90.00, 95.82, 90.00

Refinement procedure

Resolution	29.737 - 2.500
R-factor	0.2497
Rwork	0.248
R-free	0.28340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6o3a
RMSD bond length	0.002
RMSD bond angle	0.540
Data reduction software	XPREP
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.740	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.196	1.011
Rpim	0.128	0.627
Number of reflections	41997
<I/σ(I)>	7.5
Completeness [%]	98.7
Redundancy	3.3
CC(1/2)	0.975	0.659

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	293	0.2 M Na2HPO4, 20% (w/v) PEG 3350