6O2W
Crystal structure of the SARAF luminal domain Cys-lock mutant dimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 |
Unit cell lengths | 24.910, 30.790, 76.140 |
Unit cell angles | 88.58, 86.03, 89.79 |
Refinement procedure
Resolution | 37.930 - 2.101 |
R-factor | 0.1775 |
Rwork | 0.175 |
R-free | 0.23350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6o2u |
RMSD bond length | 0.007 |
RMSD bond angle | 0.934 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.1.27) |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.930 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.076 | 0.939 |
Rmeas | 0.089 | 1.095 |
Rpim | 0.046 | 0.561 |
Total number of observations | 41899 | |
Number of reflections | 11188 | 461 |
<I/σ(I)> | 12.3 | |
Completeness [%] | 85.1 | 43.9 |
Redundancy | 3.7 | 3.8 |
CC(1/2) | 0.997 | 0.609 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 3-5% glycerol or ethylene glycol |