6O2V

Crystal structure of the SARAF luminal domain Cys-lock mutant monomer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.116
Spacegroup name	P 21 21 21
Unit cell lengths	59.689, 60.953, 63.441
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.954 - 1.580
R-factor	0.1674
Rwork	0.165
R-free	0.20320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6o2u
RMSD bond length	0.006
RMSD bond angle	0.854
Data reduction software	XDS
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	63.441	63.441	1.660
High resolution limit [Å]	1.580	4.990	1.580
Rmerge		0.031	0.519
Rmeas	0.099	0.036	0.651
Rpim	0.038	0.014	0.305
Number of reflections	32028	1154	4214
<I/σ(I)>	16.2	17.3	1.5
Completeness [%]	98.7	99.9	91.5
Redundancy	6.6	6.4	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.4	293	0.1M sodium acetate, pH 4.2-4.6 and 1.2-1.6M sodium formate