6O2U
Crystal structure of the SARAF luminal domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.127 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 50.980, 63.930, 79.920 |
Unit cell angles | 90.00, 100.68, 90.00 |
Refinement procedure
Resolution | 39.432 - 1.800 |
R-factor | 0.1951 |
Rwork | 0.193 |
R-free | 0.22750 |
Structure solution method | SIRAS |
RMSD bond length | 0.007 |
RMSD bond angle | 0.934 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.2.17) |
Phasing software | SHARP |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.432 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.070 | 1.819 |
Rmeas | 0.075 | 2.013 |
Rpim | 0.028 | 0.846 |
Number of reflections | 23024 | 1167 |
<I/σ(I)> | 12.1 | |
Completeness [%] | 98.2 | 83.7 |
Redundancy | 6.9 | 5.4 |
CC(1/2) | 0.999 | 0.349 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M BisTris, pH 6.5, 18-20% PEG 5000 MME |