6O2D
Schizosaccharomyces pombe Cnp3 Cupin Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2016-06-15 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9786 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 55.160, 55.160, 206.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.074 - 2.520 |
R-factor | 0.2188 |
Rwork | 0.217 |
R-free | 0.25100 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.248 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AutoSol |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.440 | 2.590 |
High resolution limit [Å] | 2.520 | 2.520 |
Rpim | 0.016 | 0.156 |
Number of reflections | 11544 | 804 |
<I/σ(I)> | 32.9 | |
Completeness [%] | 99.6 | |
Redundancy | 13.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | Protein was mixed with 0.1 M HEPES (pH 7.0), 20% PEG 3350, and 6% w/v Trimethylamine N-oxide dihydrate in a 1:1 ratio (v/v). The reservoir solution was made up of 0.25 M potassium fluoride, 0.125 M HEPES pH 7, and 25% PEG 3350 |