6O09

Structure of AtPCNA in complex with the PIP motif of ATXR6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-08-01
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 1
Unit cell lengths	72.902, 90.549, 90.510
Unit cell angles	60.05, 73.46, 73.59

Refinement procedure

Resolution	29.652 - 2.060
R-factor	0.1972
Rwork	0.197
R-free	0.23430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zvv
RMSD bond length	0.008
RMSD bond angle	0.933
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.652	2.130
High resolution limit [Å]	2.060	2.060
Number of reflections	219871	10607
<I/σ(I)>	9.2
Completeness [%]	94.4
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		294.15	0.2 M sodium malonate 20% PEG3350