6NZI

Low resolution crystal structure of the bacterial multidrug efflux transporter AcrB in the presence of cadmium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-13
Detector	MAR CCD 165 mm
Wavelength(s)	0.97857
Spacegroup name	H 3 2
Unit cell lengths	144.280, 144.280, 519.520
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.797 - 4.440
R-factor	0.2629
Rwork	0.260
R-free	0.31990
Data reduction software	XDS
Data scaling software	xia2
Phasing software	MrBUMP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.797	4.597
High resolution limit [Å]	4.440	4.440
Number of reflections	13142	1297
<I/σ(I)>	11	3
Completeness [%]	98.5	100
Redundancy	8.9	9.3
CC(1/2)	0.998	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	Tris, pH=7.5, 250 mM choline chloride, 10% PEG 2K MME, 5 mM CdSO4