6NYP

Crystal structure of UL144/BTLA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-31
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	2.0663
Spacegroup name	P 1 21 1
Unit cell lengths	66.990, 77.200, 101.720
Unit cell angles	90.00, 91.67, 90.00

Refinement procedure

Resolution	39.930 - 2.700
R-factor	0.232
Rwork	0.230
R-free	0.27650
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.355
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.930	39.930	2.770
High resolution limit [Å]	2.700	12.080	2.700
Rmerge	0.163	0.043	3.853
Rmeas	0.164	0.044	3.889
Number of reflections	28639	608	4085
<I/σ(I)>	26.91	114.76	1.45
Completeness [%]	99.5	97	97.9
Redundancy	53.66	59.613	53.34
CC(1/2)	1.000	1.000	0.615

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	0.1 M CHES pH 9.5, 20 % W/V PEG 8000