6NYD

Crystal Structure of S. cerevisiae Ubc3 (Cdc34)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	108
Detector technology	CCD
Collection date	2016-11-28
Detector	RAYONIX MX300-HS
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	40.084, 49.022, 103.684
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.387 - 1.650
R-factor	0.1731
Rwork	0.171
R-free	0.19910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mdk
RMSD bond length	0.010
RMSD bond angle	1.072
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.710
High resolution limit [Å]	1.650	1.650
Rmerge	0.089	1.151
Rpim	0.039	0.511
Number of reflections	25422
<I/σ(I)>	20
Completeness [%]	99.8
Redundancy	6.5
CC(1/2)	0.981	0.609

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.06 M zinc acetate, 0.108mM sodium cacodylate, 14.4% PEG 8,000, 20% glycerol