6NY9

Alpha/beta hydrolase domain-containing protein 10 from mouse

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979
Spacegroup name	P 63 2 2
Unit cell lengths	99.388, 99.388, 149.597
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	56.460 - 1.660
R-factor	0.1963
Rwork	0.195
R-free	0.21880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3llc
RMSD bond length	0.011
RMSD bond angle	1.034
Phasing software	PHENIX (1.13-2998)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.460	1.717
High resolution limit [Å]	1.657	1.657
Rmerge	0.108
Number of reflections	52270	4994
<I/σ(I)>	20.82	1.6
Completeness [%]	92.8	76.98
Redundancy	12.9	12.8
CC(1/2)	0.998	0.686

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION	6.4	277.15	0.08 M strontium chloride hexahydrate, 0.04 M sodium cacodylate trihydrate pH 6.4, 25% v/v (+/-)-2-methyl-2,4-pentanediol (MPD), 0.012 M spermine tetrahydrochloride