6NY6
Structure of dimeric Escherichia coli toxin YoeB bound to the Thermus thermophilus 30S ribosome
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-12-12 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 401.710, 401.710, 175.440 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.826 - 3.740 |
R-factor | 0.2067 |
Rwork | 0.206 |
R-free | 0.22420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j5e |
RMSD bond length | 0.008 |
RMSD bond angle | 1.163 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (dev_3406) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.826 | 3.874 |
High resolution limit [Å] | 3.740 | 3.740 |
Rmerge | 0.068 | 0.485 |
Number of reflections | 145546 | 9392 |
<I/σ(I)> | 10.95 | 1.08 |
Completeness [%] | 96.9 | 85.1 |
Redundancy | 3.29 | 2.17 |
CC(1/2) | 0.999 | 0.673 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | MPD, AMMONIUM CHLORIDE, POTASSIUM CHLORIDE, MAGNESIUM ACETATE, SODIUM CACODYLATE, pH 6.5 |