6NY5

Crystal structure of the PUM-HD domain of S. pombe Puf1 in complex with RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-09
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54
Spacegroup name	I 41 2 2
Unit cell lengths	159.677, 159.677, 215.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.759 - 3.002
R-factor	0.2146
Rwork	0.211
R-free	0.26340
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AutoSol
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.050
High resolution limit [Å]	3.000	8.130	3.000
Rmerge	0.062	0.023	0.526
Rmeas	0.066	0.025	0.562
Rpim	0.023	0.009	0.197
Total number of observations	226676
Number of reflections	28120	1515	1376
<I/σ(I)>	12.4
Completeness [%]	99.9	98.1	100
Redundancy	8.1	7.3	8.1
CC(1/2)		0.999	0.960

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	1.4 M magnesium sulfate, 0.1 M MES