6NX5
Crystal structure of the RRM domain of S. pombe Puf1 in the P21 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-02-18 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.854, 37.592, 73.930 |
Unit cell angles | 90.00, 94.20, 90.00 |
Refinement procedure
Resolution | 38.222 - 1.554 |
R-factor | 0.195 |
Rwork | 0.193 |
R-free | 0.23130 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.580 |
High resolution limit [Å] | 1.550 | 4.210 | 1.550 |
Rmerge | 0.061 | 0.056 | 0.549 |
Rmeas | 0.068 | 0.063 | 0.610 |
Rpim | 0.029 | 0.029 | 0.260 |
Total number of observations | 201031 | ||
Number of reflections | 36421 | 1699 | 1655 |
<I/σ(I)> | 13.2 | ||
Completeness [%] | 98.1 | 87.1 | 90.9 |
Redundancy | 5.5 | 5 | 5 |
CC(1/2) | 0.991 | 0.843 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 15% w/v PEG3350, 0.1 M MES |