6NWY
Modified tRNA(Pro) bound to Thermus thermophilus 70S (near-cognate)
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-12 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 210.740, 450.260, 626.110 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.942 - 3.500 |
R-factor | 0.2409 |
Rwork | 0.240 |
R-free | 0.25790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4v5c |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX (1.14_3260) |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.942 | 49.942 | 3.290 |
High resolution limit [Å] | 3.200 | 14.330 | 3.200 |
Rmerge | 0.175 | 0.031 | 0.754 |
Rmeas | 0.201 | 0.035 | 0.982 |
Total number of observations | 3283538 | ||
Number of reflections | 863724 | 10860 | 43415 |
<I/σ(I)> | 6.77 | 32.11 | 1.02 |
Completeness [%] | 89.8 | 94.5 | 61.4 |
Redundancy | 3.802 | 4.641 | 1.857 |
CC(1/2) | 0.995 | 0.999 | 0.327 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 293 | 0.1 M Tris-HOAc, pH 7.6, 0.15 M L-arginine HCl, 2.9% (v/v) PEG 20K, 12% (v/v) MPD, 0.0005M BME |