6NWW
Crystal structure of the RRM domain of S. pombe Puf1 in the P212121 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-01-03 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.692, 63.896, 79.544 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.490 - 2.060 |
R-factor | 0.1776 |
Rwork | 0.172 |
R-free | 0.22510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6nx5 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.100 |
High resolution limit [Å] | 2.060 | 5.590 | 2.060 |
Rmerge | 0.080 | 0.054 | 0.206 |
Total number of observations | 63326 | ||
Number of reflections | 17513 | 888 | 838 |
<I/σ(I)> | 9.2 | ||
Completeness [%] | 97.9 | 88.4 | 95.1 |
Redundancy | 3.6 | 3.8 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.6 | 293 | 0.3 M sodium chloride, 0.1 M citric acid, 20% glycerol |