6NWH
Structures of the transcriptional regulator BgaR, a lactose sensor.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.008020 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 46.168, 86.568, 119.812 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.210 - 2.030 |
| R-factor | 0.18009 |
| Rwork | 0.179 |
| R-free | 0.20634 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.451 |
| Data reduction software | ADDREF |
| Data scaling software | Aimless |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.200 | 2.080 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.196 | 0.749 |
| Rpim | 0.040 | 0.230 |
| Number of reflections | 31671 | 2137 |
| <I/σ(I)> | 14 | 4.2 |
| Completeness [%] | 99.4 | 92.5 |
| Redundancy | 24.2 | 11.3 |
| CC(1/2) | 0.997 | 0.859 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 5 mg/mL protein; 1.33 M sodium malonate, 100 mM citrate pH 5.5 in 200 nL plus 200 nL drops at 20 C |
