6NWE
Crystal structure of bovine opsin with beta octyl glucoside bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2016-10-22 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 1.033 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 242.169, 242.169, 110.716 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.100 - 2.705 |
| R-factor | 0.1976 |
| Rwork | 0.196 |
| R-free | 0.22330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4j4q |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.842 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.100 | 2.714 |
| High resolution limit [Å] | 2.700 | 2.705 |
| Rmerge | 0.689 | |
| Rmeas | 0.756 | |
| Rpim | 0.308 | |
| Number of reflections | 33699 | 2077 |
| <I/σ(I)> | 16.6 | |
| Completeness [%] | 99.3 | 99.7 |
| Redundancy | 5.7 | 5.9 |
| CC(1/2) | 0.811 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 3.1-3.3M ammonium sulfate, 0.1M sodium acetate buffer, pH 5.5 |
