6NWD

X-ray Crystallographic structure of Gloeobacter rhodopsin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-11
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0332
Spacegroup name	C 2 2 21
Unit cell lengths	54.250, 129.250, 82.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.790 - 2.000
Rwork	0.230
R-free	0.24440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ddl
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	BALBES
Refinement software	PHENIX ((1.15.2_3472))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.790	2.072
High resolution limit [Å]	2.000	2.000
Rmerge	0.044	0.215
Rmeas	0.063	0.305
Rpim	0.044	0.215
Number of reflections	19500	19313
<I/σ(I)>	6.6
Completeness [%]	96.4
Redundancy	1.8
CC(1/2)	0.997	0.891

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.4	307	2.6-2.8 M NaH2PO4, 1,6-hexanediol, triethylene glycol, zinc acetate, n-Octyl-beta-D-Glucoside