6NW9
CRYSTAL STRUCTURE OF A TAILSPIKE PROTEIN 3 (TSP3, ORF212) FROM ESCHERICHIA COLI O157:H7 BACTERIOPHAGE CBA120
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2015-06-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | .97932 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 99.439, 66.770, 161.600 |
| Unit cell angles | 90.00, 103.58, 90.00 |
Refinement procedure
| Resolution | 54.158 - 1.850 |
| R-factor | 0.1523 |
| Rwork | 0.151 |
| R-free | 0.18390 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.357 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.14_3228:)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.158 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.048 | 0.217 |
| Number of reflections | 175535 | 17301 |
| <I/σ(I)> | 12.5 | 4.8 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 18% PEG 8000, 0.2M sodium chloride, 0.1M N-Cyclohexyl-2-aminoethanesulfonic acid (CHES) |
