6NVQ

Crystal structure of the VASH1-SVBP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-30
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.966
Spacegroup name	P 21 21 2
Unit cell lengths	70.453, 128.929, 45.222
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.610 - 2.100
R-factor	0.19839
Rwork	0.198
R-free	0.21428
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	1.220
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	CRANK2
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.610	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.062	1.538
Number of reflections	24696	3536
<I/σ(I)>	12.8	1.1
Completeness [%]	99.7	51.8
Redundancy	4.9	4.9
CC(1/2)	0.990	0.518

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.8	293	19% PEG 3000, 0.1M Tris-HCl, 0.2M calcium chloride