6NVO

Crystal structure of Pseudomonas putida nuclease MPE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.7712
Spacegroup name	P 21 21 2
Unit cell lengths	58.224, 72.995, 41.748
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.498 - 2.196
R-factor	0.2079
Rwork	0.202
R-free	0.26310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6nvp
RMSD bond length	0.009
RMSD bond angle	1.060
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXCD
Refinement software	PHENIX ((1.14_3211: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.240
High resolution limit [Å]	2.196	2.196
Rpim	0.034	0.194
Number of reflections	8980
<I/σ(I)>	27.8	4.4
Completeness [%]	93.9	72.3
Redundancy	4.6	3.3
CC(1/2)		0.925

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	293	0.1 mM Tris-HCl pH 7.2, 16% PEG MME 2000, 5mM MnCl2