6NVM
Crystal structure of 23S rRNA methyltransferase ErmE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-3 |
Synchrotron site | ESRF |
Beamline | MASSIF-3 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-12 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.9677 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 76.040, 76.040, 104.920 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.549 - 1.750 |
R-factor | 0.1935 |
Rwork | 0.192 |
R-free | 0.21850 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.586 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.549 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.093 | |
Rmeas | 0.096 | |
Rpim | 0.025 | |
Number of reflections | 31708 | 3116 |
<I/σ(I)> | 19.04 | 0.77 |
Completeness [%] | 99.9 | 99.84 |
Redundancy | 15 | |
CC(1/2) | 1.000 | 0.324 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 294 | 2% (v/v) Tacsimate pH 5.0, 0.1 M Sodium citrate tribasic dihydrate pH 5.6 and 16% (w/v) PEG 3350 |