6NUH

Non-covalent DNA-protein complex between E. coli YedK and ssDNA containing an abasic site analog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	110
Detector technology	CCD
Collection date	2018-12-04
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 1 21 1
Unit cell lengths	47.543, 44.129, 55.093
Unit cell angles	90.00, 102.34, 90.00

Refinement procedure

Resolution	39.597 - 1.594
R-factor	0.1447
Rwork	0.143
R-free	0.17730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6nua
RMSD bond length	0.008
RMSD bond angle	0.963
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.660
High resolution limit [Å]	1.590	1.600
Rmerge	0.075	0.397
Rmeas	0.086	0.455
Rpim	0.042	0.220
Number of reflections	29626	2715
<I/σ(I)>	21.2	2.6
Completeness [%]	98.5	91.1
Redundancy	4.1	4
CC(1/2)	0.990	0.869

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.1 M Bis-Tris pH 5.4, 23% (w/v) PEG 3350, 0.05 M KH2PO4