6NU9
Crystal Structure of a Zinc-Binding Non-Structural Protein from the Hepatitis E Virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-09 |
| Detector | DECTRIS PILATUS3 R 300K |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 64.399, 64.399, 158.901 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 52.624 - 1.761 |
| R-factor | 0.2385 |
| Rwork | 0.237 |
| R-free | 0.26910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | in-house model |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHENIX (1.14_3260) |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.624 | 1.820 |
| High resolution limit [Å] | 1.761 | 1.760 |
| Rmerge | 0.228 | 1.028 |
| Rpim | 0.038 | 0.334 |
| Number of reflections | 20137 | 1960 |
| <I/σ(I)> | 13.4 | |
| Completeness [%] | 99.9 | |
| Redundancy | 33.7 | |
| CC(1/2) | 0.997 | 0.339 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 18% w/v PEG3350, 180 mM lithium nitrate, 10 mM nickel chloride |
