6NSC
Crystal structure of the A/Brisbane/10/2007 (H3N2) influenza virus hemagglutinin G186V/L194P mutant apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 100.601, 100.601, 384.460 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.250 |
| R-factor | 0.19906 |
| Rwork | 0.198 |
| R-free | 0.22844 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6aoq |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.464 |
| Data scaling software | HKL-2000 (712) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rpim | 0.020 | 0.220 |
| Number of reflections | 36162 | 3556 |
| <I/σ(I)> | 29.7 | 2.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 19 | 17.1 |
| CC(1/2) | 1.000 | 0.920 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M CAPS pH 10.5 and 29% PEG 400 |
