6NS7
Crystal structure of murine caspase-11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 120.712, 121.092, 78.431 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.927 - 2.400 |
| R-factor | 0.1963 |
| Rwork | 0.194 |
| R-free | 0.24160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3e4c |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.320 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.930 | 2.486 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmeas | 0.124 | |
| Number of reflections | 86796 | 14012 |
| <I/σ(I)> | 10.62 | 1.57 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 7 | 6.9 |
| CC(1/2) | 0.998 | 0.765 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 25mM Bis-Tris, 0.2M ammonium sulfate, and 25% PEG3350 |
