6NRR
Crystal structure of Dpr11 IG1 bound to DIP-gamma IG+IG2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2014-10-06 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.96638 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 85.364, 85.364, 103.584 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.279 - 2.500 |
R-factor | 0.21 |
Rwork | 0.207 |
R-free | 0.26070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5eo9 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.699 |
Data reduction software | XDS (January 10, 2014) |
Data scaling software | XDS (January 10, 2014) |
Phasing software | PHASER (2.5.7) |
Refinement software | PHENIX (dev_3374) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.650 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.161 | 1.818 |
Rmeas | 0.168 | 1.931 |
Number of reflections | 13814 | 2138 |
<I/σ(I)> | 16.48 | 1.17 |
Completeness [%] | 99.7 | 98.1 |
Redundancy | 13.26 | 8.61 |
CC(1/2) | 0.998 | 0.577 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 294 | 0.1 M sodium citrate, pH 5.5, 2 M ammonium sulfate |