6NRO

Human parainfluenza virus type 3 fusion protein N-terminal heptad repeat domain+VIQKI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033
Spacegroup name	P 1 21 1
Unit cell lengths	39.970, 52.250, 54.110
Unit cell angles	90.00, 100.56, 90.00

Refinement procedure

Resolution	39.293 - 1.750
R-factor	0.2192
Rwork	0.217
R-free	0.24440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ztm
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.293	1.813
High resolution limit [Å]	1.750	1.750
Rmeas	0.049	1.238
Rpim	0.019	0.480
Number of reflections	22158	2189
<I/σ(I)>	18.26	1.45
Completeness [%]	99.5	99.95
Redundancy	6.6	6.5
CC(1/2)	1.000	0.726

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	30 mM NaF, 30 mM NaBr, 30 mM NaI, 20% (v/v) PEG 500 MME, 10% (w/v) PEG 20000 in 100 mM imidazole/MES monohydrate buffer (pH 6.5)20% (v/v) PEG 500 MME, 10% (w/v) PEG 20000 in 100 mM imidazole/MES monohydrate buffer (pH 6.5)