6NQZ
Flagellar protein FcpB from Leptospira interrogans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 109 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-04-28 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.735, 65.653, 134.396 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.010 - 2.580 |
| R-factor | 0.206 |
| Rwork | 0.204 |
| R-free | 0.25000 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | TRUNCATE |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | SHELXCD |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 67.232 | 37.151 | 2.720 |
| High resolution limit [Å] | 2.577 | 8.150 | 2.580 |
| Rmerge | 0.051 | 0.395 | |
| Rmeas | 0.150 | 0.061 | 0.464 |
| Rpim | 0.078 | 0.033 | 0.241 |
| Number of reflections | 17479 | 634 | 2442 |
| <I/σ(I)> | 8.1 | 11.4 | 1.9 |
| Completeness [%] | 99.2 | 99.1 | 97.1 |
| Redundancy | 3.6 | 3.1 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | (NH4)I, PEG 3350, MES, Glycerol |
