6NQV

Crystal structure of fast switching M159T mutant of fluorescent protein Dronpa (Dronpa2), Y63(3-CH3Y)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.195
Spacegroup name	P 1 21 1
Unit cell lengths	79.988, 85.729, 143.006
Unit cell angles	90.00, 94.91, 90.00

Refinement procedure

Resolution	39.344 - 2.700
R-factor	0.2567
Rwork	0.255
R-free	0.29530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uts
RMSD bond length	0.009
RMSD bond angle	1.236
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.13rc2_2986: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.344	2.770
High resolution limit [Å]	2.700	2.700
Rmeas	0.200	1.380
Number of reflections	51870	3833
<I/σ(I)>	7.7	1.6
Completeness [%]	97.4	98.4
Redundancy	7.1	7.4
CC(1/2)	0.997	0.842

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	0.1 M Tris-HCl at pH 7.4, 0.1 M MgCl2, 18% PEG 3350