6NOF

Crystal structure of FBF-2 repeat 5 mutant (C363A, R364Y, Q367S) in complex with 8-nt RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-14
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0
Spacegroup name	P 61
Unit cell lengths	96.544, 96.544, 101.066
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.211 - 2.251
R-factor	0.1685
Rwork	0.164
R-free	0.22220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3k5q
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.290
High resolution limit [Å]	2.250	6.100	2.250
Rmerge	0.104	0.043	0.772
Rmeas	0.115	0.047	0.848
Rpim	0.048	0.020	0.350
Number of reflections	25386	1291	1266
<I/σ(I)>	8.6
Completeness [%]	99.9	98.5	100
Redundancy	5.7	5.5	5.7
CC(1/2)		0.998	0.802

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	100 mM Tris pH 8.0, 15% [w/v] PEG 8000, 8% [v/v] ethylene glycol