6NNU

Xanthomonas citri Phospho-PGM in complex with glucose-1,6-phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2018-03-28
Detector	RDI CMOS_8M
Wavelength(s)	1.00003
Spacegroup name	P 21 21 21
Unit cell lengths	43.690, 54.720, 173.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.296 - 1.460
R-factor	0.1711
Rwork	0.169
R-free	0.20710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5bmn
RMSD bond length	0.005
RMSD bond angle	0.848
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.300	1.480
High resolution limit [Å]	1.460	1.460
Rmerge	0.145	2.150
Rmeas	0.151	2.286
Rpim	0.041	0.759
Number of reflections	73467	3579
<I/σ(I)>	14.4	0.8
Completeness [%]	100.0	100
Redundancy	13.3	8.8
CC(1/2)	0.999	0.345

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	22% PEG 8000, 0.2M MgCl, 0.1M HEPES