6NNQ

Non-covalent structure of SENP1 in complex with SUMO2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	293.15
Detector technology	CCD
Collection date	2016-01-31
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00
Spacegroup name	P 43 2 2
Unit cell lengths	98.732, 98.732, 101.805
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.354 - 2.621
R-factor	0.2313
Rwork	0.229
R-free	0.27660
RMSD bond length	0.002
RMSD bond angle	0.532
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.240	2.740
High resolution limit [Å]	2.620	2.620
Number of reflections	15667
<I/σ(I)>	1.7
Completeness [%]	100.0
Redundancy	9.7
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	20% PEG 6000, 0.1M HEPES, pH 7.5