6NNG
Tubulin-RB3_SLD-TTL in complex with compound DJ95
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 105.418, 157.979, 182.840 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.253 - 2.397 |
R-factor | 0.1938 |
Rwork | 0.192 |
R-free | 0.22660 |
Structure solution method | MOLECULAR REPLACEMENT |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.13_2998: ???) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.397 | 2.397 |
Number of reflections | 120100 | |
<I/σ(I)> | 20.89 | |
Completeness [%] | 99.2 | |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 293 | 6% PEG 4000, 5% Glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2 |