6NMY

A Cytokine-receptor complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-14
Detector	ADSC QUANTUM 315
Wavelength(s)	0.95370
Spacegroup name	P 21 21 21
Unit cell lengths	111.647, 157.251, 168.306
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.448 - 3.301
R-factor	0.2384
Rwork	0.236
R-free	0.27780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nkq 5uv8
Data reduction software	XDS
Data scaling software	Aimless (0.5.15)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.760	47.760	3.420
High resolution limit [Å]	3.300	12.780	3.300
Rmerge	0.175	0.027	1.728
Rmeas	0.194	0.030	1.914
Rpim	0.081	0.013	0.807
Total number of observations	248002	3883	24488
Number of reflections	45227	836	4368
<I/σ(I)>	8.8	41.1	1.1
Completeness [%]	99.8	95	99.5
Redundancy	5.5	4.6	5.6
CC(1/2)	0.996	0.999	0.310

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	277.15	Crystals were grown in 10 mM Tris pH6.8, 8% PEG 8000, 0.15 M magnesium chloride