6NMR

Blocking Fab 119 anti-SIRP-alpha antibody in complex with SIRP-alpha Variant 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-23
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.07822
Spacegroup name	P 1 21 1
Unit cell lengths	63.070, 113.560, 152.080
Unit cell angles	90.00, 91.35, 90.00

Refinement procedure

Resolution	38.009 - 2.420
R-factor	0.2902
Rwork	0.288
R-free	0.32320
RMSD bond length	0.005
RMSD bond angle	1.024
Data reduction software	HKL-2000
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.010	2.480
High resolution limit [Å]	2.420	2.420
Number of reflections	79584	5911
<I/σ(I)>	7.3	1.4
Completeness [%]	97.6	98.6
Redundancy	3.7	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	285	0.1 M Tris-HCl, 0.2 M MgCl2, 15% w/v PEG 4000