6NKS
Ternary complex crystal structure of K289M variant of DNA polymerase Beta with beta-gamma CHF analog of dGTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-21 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.607, 80.327, 55.391 |
| Unit cell angles | 90.00, 108.13, 90.00 |
Refinement procedure
| Resolution | 29.269 - 2.349 |
| R-factor | 0.1954 |
| Rwork | 0.189 |
| R-free | 0.25530 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.065 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.390 |
| High resolution limit [Å] | 2.349 | 2.349 |
| Number of reflections | 25647 | |
| <I/σ(I)> | 9 | |
| Completeness [%] | 99.8 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 16-18% PEG 3350, 50 mM Imidazole, 350 mM Sodium Acetate |
