6NKP
EphA2 LBD in complex with bA-WLA-YSKbio peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-07-27 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.916, 67.094, 93.111 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.949 - 2.033 |
| R-factor | 0.1697 |
| Rwork | 0.168 |
| R-free | 0.21320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hei |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.171 |
| Data reduction software | XDS (Jan 26, 2018) |
| Data scaling software | Aimless (0.7.2) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | PHENIX ((1.14rc2_3191: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.390 | 2.090 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Number of reflections | 24912 | 1673 |
| <I/σ(I)> | 13.2 | 2.3 |
| Completeness [%] | 98.9 | 92.3 |
| Redundancy | 7 | 5.4 |
| CC(1/2) | 0.997 | 0.714 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.09 M BIS-TRIS pH 5.5, 22.5% w/v PEG 3,350, 3% w/v 6-aminohexanoic acid |
