6NK8

C-terminal region of the Burkholderia pseudomallei OLD protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	C 2 2 21
Unit cell lengths	83.256, 105.669, 123.764
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.880 - 2.240
R-factor	0.2159
Rwork	0.214
R-free	0.26050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.908
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.300	2.310
High resolution limit [Å]	2.240	2.240
Number of reflections	26567
<I/σ(I)>	18.2
Completeness [%]	99.4
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M HEPES pH 7.5, 0.23 M MgCl2, 30% PEG 400 and 0.001 M glutathione