6NK3

Crystal structure of murine Mxra8 ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	293
Detector technology	CMOS
Collection date	2018-10-05
Detector	RDI CMOS_8M
Wavelength(s)	1.00003
Spacegroup name	I 2 2 2
Unit cell lengths	72.550, 142.700, 195.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.460 - 2.200
R-factor	0.1997
Rwork	0.199
R-free	0.22580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mj6
Data reduction software	XDS (Jan 26, 2018)
Data scaling software	Aimless (0.6.3)
Phasing software	PHENIX (1.12)
Refinement software	PHENIX (1.12)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.460	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	51479	4856
<I/σ(I)>	23.63	1.21
Completeness [%]	99.4	95.83
Redundancy	26.4	17.7
CC(1/2)	0.999	0.666

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.1 M Tris, pH 8.5, 8% PEG8000, 25% ethylene glycol