Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-17 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 44.613, 44.613, 104.343 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.430 - 2.350 |
| R-factor | 0.2086 |
| Rwork | 0.206 |
| R-free | 0.24420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q0w 1ubq |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.251 |
| Data reduction software | HKL-2000 (2000.0.98.706) |
| Data scaling software | HKL-2000 (2000.0.98.706) |
| Phasing software | PHASER (6.5.0) |
| Refinement software | PHENIX ((1.14_3211:000)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.059 | 0.302 |
| Number of reflections | 4788 | |
| <I/σ(I)> | 31.5 | 3.41 |
| Completeness [%] | 98.3 | 90.6 |
| Redundancy | 9.1 | 4.1 |
| CC(1/2) | 0.960 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2.1 M DL-Malic acid |
