6NJE

Crystal structure of the motor domain of human kinesin family member 22

Replaces:  3BFN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-10-10
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	44.530, 58.730, 116.453
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.480 - 2.200
R-factor	0.203
Rwork	0.200
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	continued refinement of model from PDB entry 3BFN. Previously solved using coordinates from PDB entry 3B6U.
RMSD bond length	0.010
RMSD bond angle	1.050
Data reduction software	DENZO
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.480	35.480	2.270
High resolution limit [Å]	2.200	9.060	2.200
Rmerge	0.121	0.022	0.840
Rmeas	0.130	0.024	0.946
Rpim	0.049	0.010	0.426
Total number of observations	109199	1614	5812
Number of reflections	16157		5812
<I/σ(I)>	16.1	53.8	2.4
Completeness [%]	99.5	98.6	95
Redundancy	6.8	5.8	4.4
CC(1/2)	0.998	1.000	0.768

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	291	3.2 M NaCl, 0.1 M Tris