6NJD

Crystal structure of RavD (residues 1-200) from Legionella pneumophila (strain Corby) complexed with Met-1 linked di-ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97736
Spacegroup name	P 21 21 2
Unit cell lengths	77.740, 102.090, 90.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.010 - 2.050
R-factor	0.2142
Rwork	0.212
R-free	0.24580
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.388
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.010	50.010	2.170
High resolution limit [Å]	2.050	6.090	2.050
Rmerge	0.056	0.044	0.568
Rmeas	0.061	0.048	0.613
Number of reflections	87704	3346	13771
<I/σ(I)>	17.67	38.87	3.6
Completeness [%]	100.0	99.7	100
Redundancy	6.975	6.83	7.033
CC(1/2)	0.999	0.998	0.934

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	289	100 mM Tris-HCl, pH 8.5, 200 mM magnesium chloride and 20% PEG3350