6NJA

Structure of WT RET protein tyrosine kinase domain at 1.92A resolution.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.1
Spacegroup name	C 1 2 1
Unit cell lengths	71.703, 70.816, 78.869
Unit cell angles	90.00, 102.05, 90.00

Refinement procedure

Resolution	39.310 - 1.920
R-factor	0.18807
Rwork	0.183
R-free	0.23400
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2ivt
RMSD bond length	0.007
RMSD bond angle	1.490
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	FFT
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.950
High resolution limit [Å]	1.920	1.920
Rmerge	0.041	0.614
Rmeas	0.049	0.729
Rpim	0.026	0.389
Number of reflections	28781	1327
<I/σ(I)>	25.32
Completeness [%]	97.2	90.5
Redundancy	3.4	3.3
CC(1/2)		0.732

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	295	2.2-2.6M Na FORMATE, 0.1m Na CITRATE pH5.5