6NIZ

Atomic structure of a fluorescent Ag8 cluster templated by a multistranded DNA scaffold

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	295.15
Detector technology	PIXEL
Collection date	2018-06-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.80
Spacegroup name	P 41 21 2
Unit cell lengths	33.290, 33.290, 62.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.540 - 0.930
R-factor	0.0827
Rwork	0.082
R-free	0.09120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.129
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.540	0.960
High resolution limit [Å]	0.930	0.930
Rmerge	0.079	0.510
Number of reflections	24092
<I/σ(I)>	23.8
Completeness [%]	100.0	100
Redundancy	22.6	12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	1.5 mM DNA, 9 mM silver nitrate, 70 mM sodium cacodylate (pH 6.0)