6NIU
Crystal structure of a human anti-ZIKV-DENV neutralizing antibody MZ4 in complex with ZIKV E glycoprotein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-12-15 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.97 |
Spacegroup name | P 1 |
Unit cell lengths | 113.530, 137.260, 136.790 |
Unit cell angles | 80.12, 65.74, 65.75 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 4.350 |
High resolution limit [Å] | 4.200 | 4.200 |
Rpim | 0.230 | |
Number of reflections | 16201 | 2954 |
<I/σ(I)> | 2.64 | |
Completeness [%] | 53.2 | |
Redundancy | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 291 | 1.26 M ammonium sulfate, 0.1 M CHES/NaOH (pH 9.5) and 0.2 M sodium chloride |