6NFU

Structure of the KcsA-G77A mutant or the 2,4-ion bound configuration of a K+ channel selectivity filter.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-25
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.987
Spacegroup name	I 4
Unit cell lengths	155.086, 155.086, 75.794
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.678 - 2.090
R-factor	0.21
Rwork	0.210
R-free	0.22360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1k4c
Data reduction software	HKL-2000 (v1.0)
Data scaling software	HKL-2000 (v1.0)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.678	34.678
High resolution limit [Å]	2.090	2.090
Number of reflections	53355
<I/σ(I)>	30.38
Completeness [%]	99.9
Redundancy	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG400, magnesium acetate, sodium acetate